A stock culture of cotton leaf curl virus from pakistan clcuvpk, was transmitted by whiteflies bemisia tabaci to seven plant species, including french bean. The generations f1, bc1, bc2 and f2 of the said cross were studied for the inheritance of clcuv resistance along with their parents. Management of tomato yellow leaf curl virus tylcv in tomato in north florida1 tim momol, steve olson, joe funderburk, and richard sprenkel2 1. Molecular identification of tobacco leaf curl disease in. Cotton leaf curl alabad virus clcuav, cotton leaf curl gezira virus clcugv, cotton leaf curl kokhran virus clcukv, cotton leaf curl multan virus. Inproving resistance to cotton leaf curl virus clcuv and. Climate change and weather fluctuations have a profound influence on the spread of cotton leaf curl virus. It is one of the economically important diseases in tropical and subtropical tobaccogrowing areas.
Pdf an overview of cotton leaf curl virus disease, persistant. Tomato yellow leaf curl virus differentially influences plant. Clcud cotton leaf curl virus disease, ssdna singlestranded deoxyribonucleic acid, clcumv cotton leaf curl multan virus, clcurv cotton leaf curl rajasthan virus, clcuav cotton leaf curl alabad virus, clcukv cotton leaf curl kokhran virus, palcuv papaya leaf curl virus, clcubv cotton leaf curl bangalore virus, clcubuv. Potato leafroll virus in potato crops agriculture and food. Tomato yellow leaf curl virus management for homeowners. Bemisia tabaci, the whitefly vector of tomato yellow leaf curl virus tylcv, seriously reduces tomato production and quality. An overview of cotton leaf curl virus disease clcud a serious threat to cotton productivity article pdf available in australian journal of crop science february 2015 with 1,547 reads. This articles factual accuracy may be compromised due to outofdate information. Potato leafroll virus plrv causes a disease of potatoes worldwide and occurs in western australia. C1 protein induces autophagy by disrupting the interaction of autophagyrelated protein 3 with glyceraldehyde 3phosphate dehydrogenases asigul ismayila, meng yanga, yakupjan haxima, yunjing wanga, jinlin lia, lu hana, yan wanga, xiyin zhenga, xiang weia, ugrappa nagalakshmib, yiguo hongc, linda hanleybowdoind, yule liua,e. Cotton leaf curl virus poses a major threat to cotton productivity in pakistan. The phloem limited positive sense rna virus infects potatoes and other members of the family solanaceae. Usda research yields cotton resistant to top 20 ag threat. Tomato yellow leaf curl virus tylcv belonging to the genus begomovirus in the family geminiviridae singlestranded dna virus with twinned icosahedral virions czosnek et al.
Researchers aim to save cotton from curl virus using wheat. Symptoms resemble those for bcmv as noted previously, but. Field evaluations of tomato yellow leaf curl virus resistant. Cotton leaf curl virus how is cotton leaf curl virus abbreviated. It has since been identified in the kona area of hawaii. Pdf cotton leaf curl virus clcuv disease in pakistan. Pdf cotton leaf curl virus disease a principle cause of. Initial screening was done under field conditions based on coefficient of infection ci, disease reaction to each genotype was. In india, chilli has been reported to be infected by several begomoviruses, namely chilli leaf curl virus chilcv, tomato leaf curl new delhi virus tolcndv and tomato leaf curl joydebpur virus khan et al. Tomato yellow leaf curl virus alters the host preferences. The present study describes the association of a distinct begomovirus species and a. Tomato yellow leaf curl virus tylcv university of florida. Potato leafroll virus plrv is a member of the genus polerovirus and family luteoviridae. Tomato yellow leaf curl virus disease springerlink.
Screening of germplasm to explore resistant source is a basic step towards the solution of this hazardous problem. The disease has since spread to other parts of the country as well as to india and china. Cotton leaf curl virus disease not only affect yield but also deteriorate fiber quality traits like. Cotton is cultivated in indian subcontinent as commercial cash crop and among the various diseases, cotton leaf curl disease clcud caused by cotton leaf curl virus clcuv has been found to be. Technology transgenic resistance to cotton leaf curl. Nov 27, 2006 three hundred and seven genotypes belonging to four cultivated and one wild species of capsicum were screened against pepper leaf curl virus peplcv causing devastating leaf curl disease of chilli capsicum annuum. Clcuv belongs to genus begomovirus of geminiviridae family and is transmitted by.
Symptoms consist of leaf curl, green mottle and blistering, and a zipperlike rugosity along the main veins. The infective cycle of cabbage leaf curl virus calcuv is. A rapid and efficient method for construction of an. Language watch edit tobacco leaf curl viruses tlcv are several species of plant pathogenic viruses in the genus begomovirus. Cotton leaf curl disease clcud, caused by cotton leaf curl burewala virus clcubv, is a major constraint for a successful production of cotton in pakistan.
Identification of host plant resistance to pepper leaf curl. Furthermore, it produces resistance against multiple forms of the virus. Virus diseases of beans fact sheet cornell university. It summarizes the techniques used for diagnosis, eradication and certification and emphasizes the problems inherent to the control of the virus insect vector, the use of pesticides and the resistance acquired by the insects, the appearance of new whitefly biotypes with previously unknown characteristics, and the complex relations between virus. Cotton leaf curl viruses clcuv are a number of plant pathogenic virus species of the family geminiviridae in asia and africa the major disease of cotton is caused by the cotton leaf curl geminivirus clcuv. Tobacco leaf curl disease tlcd is caused by begomoviruses in geminiviridae, and infected plants exhibit leaf thickening, downward leaf curling, vein swelling as well as stunting symptoms. Cotton leaf curl viral disease complex caused by cotton.
May 29, 2015 tomato yellow leaf curl virus tylcv, genus begomovirus, family geminiviridae is a global threat to agriculture 1. It turns normally healthy plants into worthless nonproducing plants and must be destroyed. Ziaurrehman m, herrmann h w, hameed u, haider m s, brown j k, 20. Oct 20, 2009 to identify host genes that show an altered regulation in response to cabbage leaf curl virus calcuv infection, a screening of transposant arabidopsis thaliana lines was carried out. Please update this article to reflect recent events or newly available information. Pdf cotton leaf curl disease, an emerging whitefly. Geminiviral diseases of cotton 127 cotton leaf curl disease a phylogenetic tree based on complete nucleotide sequences of all cotton begomoviruses is shown in figure 3.
Seed transmission has previously been reported for some rna. Tylcv originated in the middle easternmediterranean region and since then has. Symptoms of infection by the leaf curl virus gave rise to the disease name. Leaves of red raspberry become yellow, while those of black raspberry take on a darkgreen, greasy cast.
The peach potato aphid myzus persicae is regarded as the most efficient vector. We explored how tomato yellow leaf curl virus tylcv influenced the performance of its vector bemisia tabaci and a non. High levels of infection within a crop reduce returns as the virus greatly reduces tuber yield, size and marketability. Cotton leaf curl virus disease was originally identified in africa and first reported in the punjab region of pakistan in 1967. Plrv is transmitted by aphids in a persistent manner once an aphid acquires the virus it is infective for life. Oct 18, 2016 however, in the year 20012, in the burewala region of punjab province, resistant cotton varieties began to show typical symptoms of clcud. To study the inheritance pattern of resistance against the cotton leaf curl virus clcuv in cotton, the cotton leaf curl virus resistant lra5166 and clcuv susceptible s12 cotton cultivars were crossed. Mar 21, 2007 like the recently identified tomato yellow leaf curl virus, tylcv, cucurbit yellow stunting disorder virus is transmitted by the b and q biotypes of the sweet potato whitefly, bemicia tabaci. Cotton leaf curl virus need not to be afraid of youtube. Cotton is one of the most important crops of pakistan, accounting for over 60% of foreign exchange earnings. Tomato yellow leaf curl virus tylcv is one of the most wellknown tomatoinfecting begomoviruses and transmitted by bemisia tabaci. A study on the diversity of cotton leaf curl virus. Oct 31, 2008 as the nucleotide sequence of the sl is also highly conserved and particularly among begomoviruses, we hypothesised that a binary vector containing only the sl could be used for isolating, genotyping and phenotyping genetic variants of geminiviruses for intrapopulation studies and experimental evolution. This document is fact sheet pp184, one of a series of the plant pathology department, florida cooperative extension service, institute of food and agricultural sciences, university of florida.
Cotton leaf curl virus is not a single species of virus. This was an indication of a second epidemic and referred to as cotton leaf curl burewala virus which is now known as cotton leaf curl kokhran virusburewala strain clcukovbur. Transmission of tomato yellow leaf curl virus by bemisia. To date, this virus appears to be widespread in southeast queensland and in the bundaberg region. Detection and relationships of cotton leaf curl virus and allied. Tomato yellow leaf curl tomato yellow leaf curl virus tylcv in tomato, symptoms are more severe when infected at an earier age and vary somewhat among the different cultivars. New virus attacks melons, cucumbers and squash uanews. Abstractcotton leaf curling disease clcud, caused by cotton leaf curl virus clcuv is a devastating disease causes small vein thickening type symptom. Management of tomato yellow leaf curl virus tylcv in tomato. Several genes were identified to be virus responsive and one, crumpled leaf crl gene, was selected for further characterization. Genetics and genomics of cotton leaf curl disease, its. A group of whitefly transmitted ssdna viruses with ssdna satellites, causing leaf curl disease of cotton, vegetables, and ornamentals contents july 28, 20 page executive summary and contributors 1 i. These transgenic cotton plants do not produce the characteristic curling of the leaves and do not accumulate the virus. Thickening of veins, curling of leaves and formation of leaf enation can be observed at the underside of the infected leaf.
Whiteflies feed on leaves and transfer the viruses through their saliva. Molecular characterization of cotton infecting begomovirus es and. Cotton leaf curl virus how is cotton leaf curl virus. More than 15 young farmers of the district have been able to reap a good harvest from the american cotton crop which had been affected badly by a cotton leaf curl virus in early stages of. Our analysis, using a suite of bioinformatic tools applied to all publically available tylcv genome sequences, suggests that the virus probably arose somewhere in the middle east between the 1930s and 1950s and that its global spread only began in the. Cotton leaf curl is a destructive disease of cotton and several other malvaceous plant species that is transmitted by b. Leaves of infected cotton curl upward and bear leaf like enations on the underside along with vein thickening. Project methods icarda, syria, will provide the support and oversight for projects conducted within pakistan by pakistani researchers, including, but not limited to screening for cotton leaf curl virus clcuv, delivering new sources of resistance germplasm, in usable backgrounds, along with improved technology for transferring the resistance genes into adapted varieties, and establishing. Cotton leaf curl was first reported by farquharson in 1912 in native cottons grown in nigeria. Field evaluation of selected strains of cotton, gossypium barbadense l. The virus is picked up by colonising aphids during prolonged feeding on an infected plant. Development of cotton leaf curl virus clcuv diagnostic. Cucumber mosaic virus cmv can infect almost 800 plant species, but only a specific strain can cause a disease of bean and certain other legumes.
Cotton leaf curl viral disease complex caused by cotton leaf curl virus complex begomovirus, geminiviridae. Pdf an overview of cotton leaf curl virus disease clcud a. Author summary tomato yellow leaf curl virus tylcv poses a serious threat to tomato production throughout the temperate regions of the world. The present epidemic of cotton leaf curl disease clcud originated in the punjab region near the city of multan and was first reported in 1985, although it was. The leaves on infected canes are stiffly arched or curled downward. With this objective genetic material comprising 11060. Infected tomato plants initially show stunted and erect or. Bramble disease leaf curl virus penn state extension. Pakistan loses over one million bales of cotton each year due to clcuv. An exotic strain of tomato yellow leaf curl virus tylcv has recently been detected in several vegetable production areas of queensland.
Cotton leaf curl viral disease complex caused by cotton leaf. Pdf among most serious threats of last two decades with reference to cotton crop in pakistan is cotton leaf curl virus. Nov 01, 2011 more than 15 young farmers of the district have been able to reap a good harvest from the american cotton crop which had been affected badly by a cotton leaf curl virus in early stages of. Recently there has been a lot of publicity concerning the latest imported pest problem of florida agriculture tomato yellow leaf curl virus tylcv. Management of cotton leaf curl virus disease and its vector.
Leaf curl virus disease of cotton is caused by a complex of whiteflytransmitted geminiviruses circular, singlestranded dna viruses, referred to as cotton leaf curl virus clcuv complex, and one of several associated dna satellites that contribute to helper virus virulence. Recent reports have suggested that cotton leaf curl virus clcuv, a geminivirus of the genus begomovirus, may be responsible for cotton leaf curl disease in pakistan. The present epidemic of cotton leaf curl disease clcud originated in the punjab region near the city of multan and was first reported in 1985, although it was noted in this region as early as 1967. The virus nature was demonstrated by jones and mason 19261, and. This invention utilizes rna interference techniques to produce a transgenic cotton strain that is resistant to cotton leaf curl disease clcud. A novel cloning strategy for isolating, genotyping and. Chilli leaf curl palampur virus is a distinct begomovirus. Many blackberry varieties remain symptomless when infected. Review article an overview of cotton leaf curl virus disease. First detection of cotton leaf curl burewala virus and cognate cotton leaf curl multan betasatellite and gossypium darwinii symptomless alphasatellite in symptomatic luffa cylindrica in pakistan.